EFEKTIFITAS PENGIKATAN LOGAM Pb OLEH BAKTERI, Bacillus subtilis
DOI:
https://doi.org/10.31938/jsn.v4i2.87Abstract
Effectiveness of Heavy Metal (Pb) Binding of Bactery Bacillus subtilis
       The rapid development of technology and industries lead to higher environmental pollution by heavy metals, especially Pb. Reduction of heavy metals is commonly done using chemical and physical approach. Another alternative is biological technique which is more efficient due to its higher metal binding and readily available as materials. Bacillus subtilis was a potential bacterium in reducing heavy metal contamination. This study aimed to determine the B. subtilis binding effectiveness of Pb; to test the bacterial tolerance to Pb at varied concentrations; and to detect the accumulation sites of Pb within  B. subtilis. The results showed that B. subtilis was able to bind Pb with the effectiveness percentage  of 17.45%. Pb was accumulated in the cell walls.
Keyword: Bacillus subtilis, Pb binding
Â
ABSTRAK
        Pesatnya perkembangan teknologi dan industri menyebabkan pencemaran lingkungan yang lebih tinggi dengan logam berat, terutama Pb. Pengurangan logam berat yang biasanya dilakukan dengan menggunakan bahan kimia dan pendekatan fisik. Alternatif lain adalah teknik biologi yang lebih efisien karena logam yang lebih tinggi mengikat dan tersedia sebagai bahan. Bacillus subtilis adalah bakteri potensial dalam mengurangi kontaminasi logam. Penelitian ini bertujuan untuk mendeterminasi B. subtilis yang efektivitas mengikat Pb; untuk menguji toleransi bakteri terhadap Pb pada konsentrasi yang bervariasi; dan untuk mendeteksi akumulasi Pb dalam Bacillus subtilis. Hasil penelitian menunjukkan bahwa B. subtilis mampu mengikat Pb dengan efektivitas persentase 17,45%. Pb terakumulasi dalam dinding sel.
Kata Kunci : Bacillus subtilis, pengikatan PbDownloads
References
Abdelatey, L. M., W. K. B. Khalil, T. H. Ali and K. F. Mahrous. 2011. Heavy Metal Resistance and Gene Expression Analysis of Metal Resistance Genes in Gram-Positive Gram-Negative Bacteria Present in Egyptian Soils. Journal of Applied Science in Environmental Sanitation, 6(2): 201-211.
Akoto, O., T.N. Bruce and G. Darkol. 2008, Heavy Metals Pollution Profiles in Streams Serving the Owabi Reservoir. African Journal of Environmental Science and Technology, 2 (11): 354-359.
Badjoeri, M. 2008. Uji Kemampuan Bacillus megaterium Menyerap Logam Berat Merkuri. Jurnal Kimia Mulawarman, 6 (1).
Kumar, A., B. S. Bisht and V. D. Joshi. 2010. Biosorption of Heavy Metals by Four Acclimated Microbial Species, Bacillus spp., Pseudomonas spp., Staphylococcus spp. and Aspergillus niger. Journal Biology Environmental Science, 4(12): 97-108.
Issazadeh, K., M. R. Majid, K. Pahlaviani and A. Massiha. 2011. Bioremediation of Toxic Heavy Metals Pollutan by Bacillus spp. Isolated from Guilan Bay Sediments, North of Iran. International Conference on Biotechnology and Environment Management, 8.
Nur, H. S. 2005. Pembentukan Asam Organik oleh Isolat Bakteri Asam Laktat pada Media Ekstrak Daging Buah Durian (Durio zibethinus Murr.). Bioscientiae, 2 (1): 15-24.
Primaharinastiti, R., A. T. Poernomo dan N. S. Noor. 2004. Bioakumulasi Logam Berat Cu oleh Bacillus sp.. Berkas Penelitian Hayati, 10: 19-23.
Steel, R.E.D., dan J.H.Torrie. 1991. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. Gramedia Pustaka Utama, Jakarta.
Yadav, H., N. Satish, M. B. Prakash, and Maradala. 2012. Studies on Biological Removal of Plumb (Pb) by Bacillus subtilis. International Journal of Scientific and Engineering Research, 3(7).
Zulaika, E., A. Luqman, T. Arindah dan U. Sholikah. 2012. Bakteri Resisten Logam Berat yang Berpotensi sebagai Biosorben dan Bioakumulator. Seminar Nasional Waste Management for Sustainable Urban Development. Program Studi Biologi FMIPA, ITS. 21 Februari 2012, Surabaya.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Maulin Inggraini
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution - You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- ShareAlike - If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions - You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
