The Effect of Solvent Types on The Antibacterial and Cytotoxic Activity of Lino Bark Extract

Lodowik Landi Pote; Maximus M. Taek; Anggelinus Nadut; Gertreda Latumakulita

doi:10.31938/jsn.v15i3.825

The Effect of Solvent Types on The Antibacterial and Cytotoxic Activity of Lino Bark Extract

Authors

Lodowik Landi Pote Universitas Katolik Widya Mandira Kupang
Maximus M. Taek
Anggelinus Nadut
Gertreda Latumakulita

DOI:

https://doi.org/10.31938/jsn.v15i3.825

Keywords:

G. koordersiana Burret, maceration, phytochemical test, Antibacterial, cytotoxic

Abstract

The G. koordersiana Burret plant, especially its bark, is used by the Sumba community to treat wounds, ulcers, and liver. This study aims to determine the effect of solvent types on the antibacterial and cytotoxic activity of G. koordersiana Burret extract. The research methods used were maceration method, antibacterial test, and cytotoxic test of G. koordersiana Burret bark extract. The results showed that the yield of ethanol, methanol, and n-hexane extracts were 42.15%; 41.65%; and 0.62%, respectively. Ethanol and methanol extracts had antibacterial activity against E. coli and S. aureus bacteria, while n-hexane extract did not show antibacterial activity against both bacteria. Cytotoxic tests showed that the LC50 values of ethanol, methanol, and n-hexane extracts were 61.35 ppm; 758.58 ppm; and 2,494,181.19 ppm, respectively. Ethanol and methanol extracts had antibacterial activity and were toxic, while n-hexane extract did not show antibacterial activity and was not toxic. The antibacterial and cytotoxic activities of ethanol and methanol extracts showed the presence of secondary metabolite compounds of the alkaloid, phenolic, flavonoid, tannin, triterpenoid, and saponin groups, while n-hexane extract contained triterpenoid compounds that had no inhibitory power against bacteria or cytotoxic activity.

Downloads

Download data is not yet available.

References

Aisy, R. R., Putri, S. H., & Yuliana, T. (2022). Effect of solvents on antibacterial activity pine needle extracts against Staphylococcus aureus and application in solid soap. Jurnal Pangan Dan Agroindustri, 10(1), 47–56.

Alara, O. R., Abdurahman, N. H., & Ukaegbu, C. I. (2021). Extraction of phenolic compounds: A review. Current Research in Food Science, 4(April), 200–214. https://doi.org/10.1016/j.crfs.2021.03.011

Badaring, D. R., Sari, S. P. M., Nurhabiba, S., Wulan, W., & Lembang, S. A. R. (2020). Uji Ekstrak Daun Maja (Aegle marmelos L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Indonesian Journal of Fundamental Sciences, 6(1), 16–26. https://doi.org/10.26858/ijfs.v6i1.13941

Dai, L. I., Pote, L. L., Tukan, G. D., & Taek, M. M. (2023). Aktivitas Antioksidan dan Sitotoksik Ekstrak Etanol dan Diklorometan Kulit Batang Halay (Alstonia spectabilis R. Br). Jurnal Sains Dan Edukasi Sains, 6(2), 104–110. https://doi.org/10.24246/juses.v6i2p104-110

Febrina et al. (2015). Optimalisasi ekstraksi dan uji metabolit sekunder tumbuhan libo (Ficus variagate Blume). J. Trop. Pharm. Chem, 3(2), 74–81.

Fiana, F. M., Kiromah, N. Z. W., & Purwanti, E. (2020). Antibacterial Activity of Ethanol Extract of Breadfruit Leaf (Artocarpus altilis) Against Staphylococcus aureus and Escherichia coli Bacteria. Pharmacon: Jurnal Farmasi Indonesia, 10–20. https://doi.org/10.23917/pharmacon.v0i0.10108

Fransiska, A. N., Masyrofah, D., Marlian, H., Sakina, I. V., & Tyasna, P. S. (2021). IDENTIFIKASI SENYAWA TERPENOID DAN STEROID PADA BEBERAPA TANAMAN MENGGUNAKAN PELARUT N-HEKSAN. 2(6), 733–745. https://doi.org/https://doi.org/10.46799/jhs.v2i6.180

Hesti Marliza, Yunisa Friscia Yusri, & Henny Rachdiati. (2022). Uji Aktivitas Sitotoksik Ekstrak Kulit Bawang Putih (Allium satovum L.) Dengan Metode BSLT (Brine Shrimp Lethality Test). Jurnal Ilmiah Kedokteran Dan Kesehatan, 1(1), 216–225. https://doi.org/10.55606/klinik.v1i1.2824

Ilham, I., Supriningrum, R., & Warnida, H. (2024). Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Labu Kuning (Cucurbita moschata) Berdasarkan Perbedaan Konsentrasi Pelarut Secara Spektrofotometri UV-Vis. Chemica: Jurnal Ilmiah Kimia Dan Pendidikan Kimia, 25(1), 110. https://doi.org/10.35580/chemica.v25i1.59074

Julianti, W. P., Ikrawan, Y., & Iwansyah, A. C. (2019). Pengaruh Jenis Pelarut Terhadap Kandungan Total Fenolik, Aktifitas Antioksidan Dan Toksisitas Ekstrak Buah Ciplukan (Physalis angulata L). Jurnal Riset Teknologi Industri, 13(1), 70. https://doi.org/10.26578/jrti.v13i1.5032

Karmilah, Reymon, Nur Saadah Daud, Esti Badia, Agung Wibawa Mahatva Yodha, Muh. Azdar Setiawan, Selfyana Austin Tee, & Musdalipah. (2023). Antibacterial Activity of Meistera chinensis Rhizome toward Staphylococcus aureus ATCC 25023 and Eschericia coli ATCC 35218 by Agar Diffusion. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 8(1), 10–18. https://doi.org/10.24002/biota.v8i1.5651

Kemit, N., Widarta, I. W. R., & Nocianitri, K. A. (2016). Pengaruh Jenis Pelarut dan Waktu Maserasi Terhadap Kandungan Senyawa Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Alpukat ( Persea Americana Mill ). Jurnal Ilmu Teknologi Pangan, 5(2), 130–141.

Kirana Jati, N., Tri Prasetya, A., & Mursiti, S. (2019). Isolasi, Identifikasi, dan Uji Aktivitas Antibakteri Senyawa Alkaloid pada Daun Pepaya Info Artikel. Jurnal MIPA, 42(1), 1–6.

Kusuma, A. E., & Aprileili, D. A. (2022). Pengaruh Jumlah Pelarut Terhadap Rendemen Ekstrak Daun Katuk (Sauropus androgynus L. Merr). SITAWA : Jurnal Farmasi Sains Dan Obat Tradisional, 1(2), 125–135. https://doi.org/10.62018/sitawa.v1i2.22

Luviana, A., Putri, A., Reynaldi, R., Rahmawati, P., Azzahra, R. C., Sihombing, R. P., & Paramitha, T. (2023). Pengaruh Pelarut yang Digunakan terhadap Hasil Ekstraksi Kunyit (Curcuma Longa L.). Industrial Research Workshop and National Seminar, 123–127.

Marliza, H., & Oktaviani, D. (2021). Uji Sitotoksik Ekstrak Etanol Daun Kemumu (Colacasia Gigantea Hook.F) dengan Metode Brine Shrimp Lethality Test (BSLT). Bencoolen Journal of Pharmacy, 1(1), 38–45. https://doi.org/10.33369/bjp.v1i1.15593

Mayuna, A. A. I., Udayani, N. N. W., Dharma, N. M., Suena, S., Shantini, Sanjiwani, N. M. S., & Yani, N. K. M. S. D. (2023). Studi literatur : Pengaruh penggunaan jenis pelarut terhadap kandungan metabolit sekunder Alkaloid, Flavonoid, Tannin dan Saponin ekstrak rimpang kunyit hitam (Curcuma caesia Roxb.). Emasains : Jurnal Edukasi Matematika Dan Sains, 12(2), 115–120. https://doi.org/10.59672/emasains.v12i2.3100

Mubarokah, A., Kurniawan, & Kusumaningtyas, N. M. (2023). Penetapan Kadar Senyawa Flavonoid Ekstrak Etanol 96%, Metanol 96%, Etil Asetat 96% Rimpang Lengkuas Merah (Alpinia purpurata K.Schum) Dengan Spektrofotometri Uv-Vis. Jurnal Ilmiah Global Farmasi, 1(1), 1–8.

Mukhriani, M., Syahrana, N. A., Dhuha, N., & Ridwan, D. A. (2023). Pengaruh Penggunaan Pelarut terhadap Skrining Fitokimia dan Profil Kromatografi Lapis Tipis (KLT) Ekstrak Daun Tobo-Tobo (Ficus septica Burm. F). Jurnal Farmasi UIN Alauddin Makassar, 11(2), 7–13. https://doi.org/10.24252/jfuinam.

Ningsih, D. S., Henri, Roanisca, O., & Gus Mahardika, R. (2020). Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.). Biotropika: Journal of Tropical Biology, 8(3), 178–185. https://doi.org/10.21776/ub.biotropika.2020.008.03.06

Nugraha, A. C., Prasetya, A. T., & Mursiti, S. (2017). Isolasi, Identifikasi, Uji Aktivitas Senyawa Flavonoid sebagai Antibakteri dari Daun Mangga. Indonesian Journal of Chemical Science, 6(2), 91–96.

Pangisian, J., Sangi, M. S., & Kumaunang, M. (2022). Analisis Senyawa Metabolit Sekunder dan Uji Aktivitas Antioksidan serta Antibakteri Biji Buah Pangi (Pangium edule Reinw). Jurnal LPPM Bidang Sains Dan Teknologi, 7(1), 11–19.

Pawarti, N., Iqbal, M., Ramdini, D. A., & Yuliyanda, C. (2023). Pengaruh Metode Ekstraksi Terhadap Persen Rendemen dan Kadar Fenolik Ekstrak Tanaman yang Berpotensi sebagai Antioksidan. Medula, 13(4), 590–593.

Pote, L. L., Taek, M. M., Nadut, A., & Latumakulita, G. (2024). Pengaruh Jenis Pelarut Terhadap Kadar Senyawa Metabolit Sekunder Dan Aktivitas Antioksidan Ekstrak Kulit Batang Lino (Grewia koordersiana Burret). Akta Kimia Indonesia, 9(1), 70–90. https://doi.org/10.12962/j25493736.v9i1.20177

Putri, J. Y., Nastiti, K., & Hidayah, N. (2023). Pengaruh Pelarut Etanol 70% Dan Metanol Terhadap Kadar Flavonoid Total Ekstrak Daun Sirsak (Annona muricata Linn). Journal Pharmaceutical Care and Sciences, 3(2), 20–29. https://doi.org/10.33859/jpcs.v3i2.235

Qamar, M., Akhtar, S., Ismail, T., Wahid, M., Barnard, R. T., Esatbeyoglu, T., & Ziora, Z. M. (2021). The chemical composition and health-promoting effects of the grewia species—a systematic review and meta-analysis. Nutrients, 13(12). https://doi.org/10.3390/nu13124565

Rahmawati, J. E., Wati, A., & Handayani, S. (2024). Uji Aktivitas Sitotoksik Fraksi Daun Matoa (Pometia pinnata) dengan Metode Brine Shrimp Lethality Test (BSLT). Makassar Pharmaceutical Science Journal, 2(1), 99–112. https://doi.org/10.31957/jbp.1358

Rahmi, N., Salim, R., Miyono, & M. Ikhwan Rizki. (2021). The Effect of Solvents and Extraction Methods on Antibacterial and Free Radical Scavenging Activities from Bangkal (Nauclea subdita) Bark Extracts. Penelitian Hasil Hutan, 39(1), 13–26.

Rizal, S., & Dewi. (2015). Effect of Solvent Types On Antibacterial Activity of Bintaro (Cerbera mangas L.) Meat and Seeds Extract. Jurnal Teknologi Industri & Hasil Pertanian, 20(1), 51–64.

Safriani, L., Nasution, M. P., Munandar, H., Rahayu, Y. P., Nasution, M. P., Studi, P., Farmasi, F., Nusantara, M., Washliyah, A., Garu, J., & No, I. I. (2023). Pengaruh Metode Ekstraksi Terhadap Efek Sitotoksisitas Daun Jamblang (Syzygium Cumini L.) Pada Larva Udang Artemia salina Leach Dengan Metode Brine Shrimp Lethality Test. Jurnal Farmasi, Sains, Dan Kesehatan, 3(1), 87–101.

Salempa, P., Harfiana, G. A., Pertiwi, D. E., & Akbar, M. (2023). Phytochemical Test and Toxicity Test for Secondary Metabolite Compound Kratom Stem Ethyl Acetate Extract (Mytragyna speciosa Korth). Jurnal Chemica, 24(2), 19–25.

Sangat, H. M., Zuhud, E. A. M., & Damayanti, E. K. (2000). Kamus Penyakit dan Tumbuhan Obat Indonesia (Etnofitomedika) 1. Yayasan Obor Indonesia.

Saputri, D. R., Listyadevi, Y. L., & Damayanti, D. (2023). JURNAL INTEGRASI PROSES Website : http://jurnal.untirta.ac.id/index.php/jip PENGARUH LAMA PERENDAMAN , KONSENTRASI DAN JENIS PELARUT TERHADAP ANTOSIANIN DARI EKSTRAK BUNGA TELANG ( CLITORIA TERNATEA ) Program Studi Teknik Kimia , Jurusan Teknologi Produks. 12(1), 1–5.

Septiani, S., Dewi, E. N., & Wijayanti, I. (2017). Antibacterial Activities of Seagrass Extracts (Cymodocea rotundata) Against Staphylococcus aureus and Escherichia coli. SAINTEK PERIKANAN : Indonesian Journal of Fisheries Science and Technology, 13(1), 1. https://doi.org/10.14710/ijfst.13.1.1-6

Sri Natalia Saragih, M Pandapotan Nasution, Haris Munandar Nasution, & Rafita Yuniarti. (2023). Phytochemical Screening and Cytotoxicity Test of Daun Ungu (Graptophyllum pictum (L.) Griff) Ethanol Extract With Bslt Method. FARMASAINKES: JURNAL FARMASI, SAINS, Dan KESEHATAN, 2(2), 170–177. https://doi.org/10.32696/fjfsk.v2i2.1888

Taek, M. M., Ew, B. P., & Agil, M. (2018). Plants used in traditional medicine for treatment of malaria by Tetun ethnic people in West Timor Indonesia. Asian Pacific Journal of Tropical Medicine, 11(11), 630–637. https://doi.org/10.4103/1995-7645.246339

Ugrasena, P. Y., Puspitasari, D. R. A., & Rupayantini, D. A. (2022). Perbandingan uji sitotoksik fraksi N-heksan, fraksi etilasetat dan ekstrak purifikasi herba sambiloto (Andrographis paniculata (Burm.f.) Nees) dengan metode Brine Shrimp Lethality Test (BSLT). Journal Pharmactive, 1(1), 1–6.

Wiranata, I. G., & Sasadara, M. M. V. (2022). Effect of Solvent And Extraction Method on Secondary Metabolites And LC50 of Beetroot Extract (Beta vulgaris L.). Usadha, 2(1), 7–13. https://doi.org/10.36733/usadha.v2i1.5277