Authentication of Jamu from Paracetamol Adulteration Using Thin-Layer Chromatography And UV-Vis Spectrophotometry

Mamay Maslahat; Reggiana Nurul Ikhsani Sandy; Srikandi

doi:10.31938/jsn.v16i2.921

Authentication of Jamu from Paracetamol Adulteration Using Thin-Layer Chromatography And UV-Vis Spectrophotometry

Authors

Mamay Maslahat SCOPUS ID: 57189219858 (Analytical Chemistry) Department of Chemistry, Universitas Nusa Bangsa
Reggiana Nurul Ikhsani Sandy
Srikandi

DOI:

https://doi.org/10.31938/jsn.v16i2.921

Keywords:

industrial areas, jamu, paracetamol, TLC, UV-Vis spectrophotometry

Abstract

The distribution of herbal medicines containing chemical substances (BKO) that are dangerous for the public is prohibited based on the Regulation of the Minister of Health of the Republic of Indonesia No. 007 of 2012, however, they are still often found in the community. The types of BKO found in herbal medicines for aches and pains include dexamethasone, phenylbutazone and paracetamol. Paracetamol is an active substance used in modern medicine that has analgesic and antipyretic properties, so it can relieve pain in certain doses. Paracetamol in herbal medicines can be identified qualitatively using thin layer chromatography (TLC) with a standard paracetamol reference solution that has an Rf value of 0.77 with a mobile phase of ethyl acetate-ethanol-ammonia (85:10:5, v/v), and a UV-Vis Spectrophotometer. Therefore, authentication of herbal medicines in circulation is needed as a guarantee of safety to the public. The purpose of this study was to detect BKO paracetamol in herbal medicines for aches and pains qualitatively and quantitatively using TLC and a UV-Vis spectrophotometer. The research stages included sampling herbal medicine from three industrial areas, namely Sentul, Cakung, and Cikarang with the types of herbal medicine selected being packaged and bulk as well as powder and liquid forms; identification of paracetamol using TLC; and analysis of paracetamol using a UV-Vis Spectrophotometer. Based on the TLC analysis, it was found that 3 out of 24 samples of herbal medicine for aches and pains were positive for containing paracetamol. The three samples were obtained from the Cikarang Industrial Area with liquid preparations from branded and bulk packaging types with the codes C1Xl, C2Xl, and C2Yl. The paracetamol concentrations in these samples were 3.8417; 2.1390; and 15.1980 mg/L, respectively.

Downloads

Download data is not yet available.

Author Biography

Mamay Maslahat, SCOPUS ID: 57189219858 (Analytical Chemistry) Department of Chemistry, Universitas Nusa Bangsa

References

Badan Litbang Kesehatan. (2010). Hasil riset kesehatan dasar (Riskesdas) 2010 (Vol. 53, Issue 9). Badan Litbang Kesehatan Kementrian Kesehatan Republik Indonesia.

Badan Pengawas Obat Dan Makanan Republik Indonesia. (2023). Peraturan Badan Pengawas Obat Dan Makanan Nomor 25 Tahun 2023 Tentang Kriteria Dan Tata Laksana Registrasi Obat Bahan Alam.

Bali, S. Serawaidi, N. P. A., Aprilia, S. (2023). Validasi dan Penentuan Kadar Paracetamol dalam Jamu Pegal Linu yang Beredar di Pekanbaru Secara Spektrofotometri UV-Vis; Berkala Ilmiah Kimia Farmasi, 10 (1): 17–21.

Direktorat Pengawasan Obat Tradisional dan Suplemen Kesehatan. (2023). Laporan Tahunan Tahun 2022. Direktorat Pengawasan Obat Tradisional dan Suplemen Kesehatan. Jakarta.

Direktorat Intelejen Obat dan Makanan. (2025). Ancaman Tersembunyi di Balik Khasiat Instan - Mengupas Tuntas Bahaya Bahan Kimia Obat atau BKO dalam Obat Tradisional. https://intelijen.pom.go.id/hot-issue.

Ganea, Q. A., Bilqis, D. M., Farpina, E. (2025). Identifikasi Kandungan Parasetamol pada Jamu Pegal Linu yang Dijual di Depot Jamu Kota Samarinda. Jurnal Kesehatan Tambusai. 6(2). 9062-9071.

Harimurti, S., Ulandari, S., Widada, H., & Damarwati, V. L. (2020). Identifikasi Parasetamol dan Asam Mefenamat pada Jamu Pegel Linu dan Asam Urat yang Beredar di Daerah Istimewa Yogyakarta. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 5(2). https://doi.org/10.20961/jpscr.v5i2.41929.

Husain, F., Ysrafil, Y., Daud, R. P. A., Aisyah, A. N., Fadri, A., Nur, S. (2023). Authentication of Medicinal Chemicals in Traditional Herbal Products (Jamu) by UV-Vis Spectrophotometry Hacettepe. University Journal of the Faculty of Pharmacy. 43(2): 100-110.

Indriatmoko, D., Rudiana, T., & Saefullah, A. (2019). Analisis Kandungan Parasetamol pada Jamu Pegal Linu yang diperoleh dari Kawasan Industri Kecamatan Kibin Kabupaten Serang. Journal Itekimia, 5(1), 33–47.

Jayanti, R., Aprilia, H. & Lukmayani, Y. (2015). Analisis Bahan Kimia Obat (BKO) Glibenklamid dalam Sediaan Jamu Diabetes yang Beredar di Pasaran. Prosiding Penelitian Spesia hal 649–653.

Nurcholis W., Arianti R. (2024). Jamu sebagai Warisan Budaya Indonesia dan Inovasi Kesehatan Modern. Jurnal Jamu Indonesia. 9(1): 1–2.

Permatasari, D.A. I, Setyowati, R., Mahardika, M. P. (2022). Analisis Kualitatif dan Kuantitatif Cemaran Parasetamol pada Obat Tradisional Pegal Linu. JFSP. 8 (1): 48-59.

Pratiwi, R.A. dan Nandiyanto, A. B. D. (2022). How to Read and Interpret UV-VIS Spectrophotometric Results in Determining the Structure of Chemical Compounds. Indonesian Journal of Educational Research and Technology. 2(1): 1-20.

Rahayu, N. (2014). Analisis Bahan Kimia Obat Dalam Jamu Reumatik menggunakan Metode Kromatografi Lapis Tipis dan Spektrofotometri. KTI. Jurusan Farmasi Politeknik Kesehatan Bandung: Bandung.

Rosamah, E. (2019). Kromatografi Lapis Tipis: Metode Sederhana dalam Analisis Kimia Tumbuhan Berkayu. Samarinda: Mulawarman University Press.

Rusmalina, S., Khasanah, K., & Nugroho, D. K. (2020). Deteksi Asam Mefenamat pada Jamu Pegel Linu yang beredar di Wilayah Pekalongan. Pharmacon: Jurnal Farmasi Indonesia. https://doi.org/10.23917/pharmacon.v0i0.10111.

Saad, M. dan Flament, J. (2024). Paracetamol overdose causing acute kidney injury without hepatotoxicity: a case report. International Journal of Emergency Medicine. 17:81.

Sahumena, M.H., Ruslin, Asriyanti, Djuwarno, E. N. (2020). Identifikasi Jamu Yang Beredar Di Kota Kendari Menggunakan Metode Spektrofotometri Uv-Vis. Journal Syifa Sciences and Clinical Research. 2 (2). 65-72.

Sudewi, N. K. A. P. A., Budiartha, I. N. P., & Ujianti, N. M. P. (2020). Perlindungan Hukum Badan Pengawas Obat Dan Makanan (BPOM) Terhadap Peredaran Produk Jamu Yang Mengandung Bahan Kimia Obat Berbahaya. Jurnal Analogi Hukum,

2(2). https://doi.org/10.22225/ah.2.2.1928.246-251.

Wahyuningsih E. dan Dessidianti, R. (2022). Aplikasi FT-IR ATR Spektroskopi untuk Identifikasi Parasetamol pada Jamu Sediaan Serbuk. Camellia. 1 (2).56-60.