Usnic Acid Isolated from Lichen Usnea sp. from Ciwidey, West Java

Main Article Content

Dewi Meliati Agustini
Riga Riga
Sari Purbaya
Eva Selviana
Muhammad Habibul Ikhsan

Abstract

Indonesia is a tropical country that has a variety of plants. Plants are one potential source of medicine. One of them is Lichen Usnea sp., which is used by the community to treat several diseases, so further research is needed to study the chemical composition contained and other potentials of this plant. Research on the chemical constituent of Lichen Usnea sp. consists of various stages: sample preparation, maceration using methanol, fractionation by Vacum Liquid Chromatography (VLC) and radial chromatography guided by Thin Layer Chromatography (TLC) eluting with n-hexane, chloroform and ethyl acetate obtaining an isolated compound. The isolated compound was analyzed by various spectroscopic data (UV-Vis, FT-IR, 1H-NMR and 13C-NMR). Based on the spectra, the isolated compound was usnic acid.

Keywords:  Usnic acid;  Lichen; Usnea sp.

ABSTRAK

Isolat Asam Usnat dari Lichen Usnea sp. Asal Ciwidey, Jawa Barat

Indonesia adalah negara tropis dengan banyaknya spesies tumbuhan. Tumbuhan adalah salah satu sumber bahan baku yang potensial untuk obat–obatan. Salah satunya Lichen Usnea sp. yang digunakan masyarakat dalam pengobatan beberapa penyakit, sehingga diperlukan penelitian lebih lanjut untuk mengetahui senyawa kimia yang terkandung dan potensi lainnya dari tanaman ini. Penelitian kandungan kimia dari Lichen Usnea sp. asal Ciwidey, Jawa Barat, terdiri dari berbagai tahapan, yaitu, persiapan sampel, maserasi dengan pelarut metanol, fraksinasi dan pemurnian dengan metode Kromatografi Cair Vakum (KCV) dan kromatografi radial yang dipandu dengan Kromatografi Lapis Tipis (KLT) dengan eluen n-heksana, kloroform dan etil asetat dan menghasilkan satu senyawa. Senyawa yang diperoleh tersebut dianalisis dengan berbagai data spektroskopi (UV, IR, 1H-NMR, dan 13C-NMR). Hasil karakterisasi menunjukkan bahwa senyawa tersebut adalah asam usnat.

Kata kunci: Asam usnat; Lichen; Usnea sp.

Article Details

How to Cite
Agustini, D. M., Riga, R., Purbaya, S., Selviana, E., & Ikhsan, M. H. (2023). Usnic Acid Isolated from Lichen Usnea sp. from Ciwidey, West Java. JURNAL SAINS NATURAL, 13(1), 14–20. https://doi.org/10.31938/jsn.v13i1.463
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Articles

References

Azis, T., Hadijah Sabarwati, S., & Nurdin, M. (2014). Isolasi dan identifikasi senyawa (-)-asam usnat dari lichen usnea sp. serta aktivitas sitotoksiknya terhadap sel murine leukimia P388. Jurnal Ilmu Kefarmasian Indonesia, 13(1), 40–44.

Choudhary, M. I., Azizuddin, Jalil, S., & Atta-ur-Rahman. (2005). Bioactive phenolic compounds from a medicinal lichen, Usnea longissima. Phytochemistry, 66(19), 2346–2350.

Galinato, M. (2018). Review of the lichen genus Usnea in the Philippines. Studies in Fungi, 3(1), 39–48.

Jannah, M., A`yun, Q., Afifah, N., Prasetya, E., & Hariri, M. R. (2022). Usnea in west java : a potential source of bioactive secondary metabolites. Berkala Penelitian Hayati, 28(1), 26–31.

Maulidiyah, M., Darmawan, A., Ahmad, E., Musdalifah, A., Wibowo, D., Salim, L. O. A., Arham, Z., Mustapa, F., Nurdin, I. F. A., & Nurdin, M. (2021). Antioxidant activity-guided isolation of usnic acid and diffractaic acid compounds from lichen genus Usnea sp. Journal of Applied Pharmaceutical Science, 11(2), 075–083.

Maulidiyah, M., Darmawan, A., Wahyu, W., Musdalifah, A., Salim, L. O. A., & Nurdin, M. (2022). Potential of usnic acid compound from lichen genus Usnea sp. as antidiabetic agents. Journal of Oleo Science, 71(1), 127–134.

Millot, M., Kaouadji, M., Champavier, Y., Gamond, A., Simon, A., & Chulia, A. J. (2013). Usnic acid derivatives from Leprocaulon microscopicum. Phytochemistry Letters, 6(1), 31–35.

Oh, J. M., Kim, Y. J., Gang, H. S., Han, J., Ha, H. H., & Kim, H. (2018). Antimicrobial activity of divaricatic acid isolated from the Lichen Evernia mesomorpha against methicillin-resistant Staphylococcus aureus. Molecules, 23(12).

Petrová, K., BaÄkorová, M., DemÄišáková, Z., Petrovová, E., Goga, M., Vilková, M., Frenák, R., BaÄkor, M., MojžiÅ¡, J., & Kello, M. (2022). Usnic acid isolated from Usnea antarctica (Du Rietz) reduced in vitro angiogenesis in VEGF- and bFGF-stimulated HUVECs and ex ovo in quail chorioallantoic membrane (CAM) Assay. Life, 12(9).

Popovici, V., Bucur, L. A., Schröder, V., Gherghel, D., Mihai, C. T., Caraiane, A., Badea, F. C., Vochița, G., & Badea, V. (2020). Evaluation of the cytotoxic activity of the Usnea barbata (L.) F. H. Wigg dry extract. Molecules, 25(8).

Salgado, F., Albornoz, L., Cortéz, C., Stashenko, E., Urrea-Vallejo, K., Nagles, E., Galicia-Virviescas, C., Cornejo, A., Ardiles, A., Simirgiotis, M., García-Beltrán, O., & Areche, C. (2018). Secondary metabolite profiling of species of the genus Usnea by UHPLC-ESI-OT-MS-MS. Molecules, 23(1), 1–16.

Sepahvand, A., Studzińska-Sroka, E., Ramak, P., & Karimian, V. (2021). Usnea sp.: Antimicrobial potential, bioactive compounds, ethnopharmacological uses and other pharmacological properties; a review article. Journal of Ethnopharmacology, 268(July 2020).

Van Nguyen, K., Duong, T. H., Nguyen, K. P. P., Sangvichien, E., Wonganan, P., & Chavasiri, W. (2018). Chemical constituents of the lichen Usnea baileyi (Stirt.) Zahlbr. Tetrahedron Letters, 59(14), 1348–1351.

Vu, T. H., Lamer, A. C. Le, Lalli, C., Samson, J. B. M., Dévéhat, F. L. Le, & Seyec, J. Le. (2015). Depsides: Lichen metabolites active against hepatitis C virus. PLoS ONE, 10(3), 1–14.

Yu, X., Guo, Q., Su, G., Yang, A., Hu, Z., Qu, C., Wan, Z., Li, R., Tu, P., & Chai, X. (2016). Usnic acid derivatives with cytotoxic and antifungal activities from the Lichen Usnea longissima. Journal of Natural Products, 79(5), 1373–1380.